Evaluation of bisulfite kits for DNA methylation profiling in terms of DNA fragmentation and DNA recovery using digital PCR

DNA methylation is one of the most important epigenetic modifications in the regulation of gene transcription. The current gold standard to study this modification is bisulfite sequencing. Although multiple commercial bisulfite treatment kits provide good conversion efficiencies, DNA loss and especi...

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Udgivet i:PLoS One
Main Authors: Kint, Sam, De Spiegelaere, Ward, De Kesel, Jonas, Vandekerckhove, Linos, Van Criekinge, Wim
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2018
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6002050/
https://ncbi.nlm.nih.gov/pubmed/29902267
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0199091
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