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Directed evolution mimics allosteric activation by stepwise tuning of the conformational ensemble
Allosteric enzymes contain a wealth of catalytic diversity that remains distinctly underutilized for biocatalysis. Tryptophan synthase is a model allosteric system and a valuable enzyme for the synthesis of non-canonical amino acids (ncAA). Previously, we evolved the β-subunit from Pyrococcus furios...
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| Publicado no: | J Am Chem Soc |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5999571/ https://ncbi.nlm.nih.gov/pubmed/29712420 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jacs.8b03490 |
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