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DNA supercoiling measurement in bacteria
DNA supercoiling plays critical roles in several essential DNA metabolic pathways, such as replication, transcription and recombination. Typically plasmid DNA molecules are used to measure DNA supercoiling status inside bacterial cells. In this chapter, we describe how to isolate plasmid DNA molecul...
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| Publicado no: | Methods Mol Biol |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5995477/ https://ncbi.nlm.nih.gov/pubmed/29177733 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-7459-7_4 |
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