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Biochemical and biophysical properties of positively supercoiled DNA

In this paper we successfully developed a procedure to generate the (+) supercoiled (sc) plasmid DNA template pZXX6 in the milligram range. With the availability of the (+) sc DNA, we are able to characterize and compare certain biochemical and biophysical properties of (+) sc, (−) sc, and relaxed (...

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Publicat a:Biophys Chem
Autors principals: Liu, Yingting, Berrido, Andrea, Hua, Zi-Chun, Tse-Dinh, Yuk-Ching, Leng, Fenfei
Format: Artigo
Idioma:Inglês
Publicat: 2017
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5773249/
https://ncbi.nlm.nih.gov/pubmed/28887044
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpc.2017.08.008
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