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Biochemical and biophysical properties of positively supercoiled DNA
In this paper we successfully developed a procedure to generate the (+) supercoiled (sc) plasmid DNA template pZXX6 in the milligram range. With the availability of the (+) sc DNA, we are able to characterize and compare certain biochemical and biophysical properties of (+) sc, (−) sc, and relaxed (...
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| Publié dans: | Biophys Chem |
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| Auteurs principaux: | , , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
2017
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5773249/ https://ncbi.nlm.nih.gov/pubmed/28887044 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpc.2017.08.008 |
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