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The Combinational Use of CRISPR/Cas9 and Targeted Toxin Technology Enables Efficient Isolation of Bi-Allelic Knockout Non-Human Mammalian Clones

Recent advances in genome editing systems such as clustered regularly interspaced short palindromic repeats/CRISPR-associated protein-9 nuclease (CRISPR/Cas9) have facilitated genomic modification in mammalian cells. However, most systems employ transient treatment with selective drugs such as purom...

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Bibliografische gegevens
Gepubliceerd in:Int J Mol Sci
Hoofdauteurs: Watanabe, Satoshi, Sakurai, Takayuki, Nakamura, Shingo, Miyoshi, Kazuchika, Sato, Masahiro
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: MDPI 2018
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5979347/
https://ncbi.nlm.nih.gov/pubmed/29617297
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms19041075
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