A single blastocyst assay optimized for detecting CRISPR/Cas9 system-induced indel mutations in mice

BACKGROUND: Microinjection of clustered regulatory interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9)-related RNA and DNA into fertilized eggs is a novel approach for creating gene-modified mice. Blastocysts obtained just before implantation may be appropriate for testi...

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Hauptverfasser: Sakurai, Takayuki, Watanabe, Satoshi, Kamiyoshi, Akiko, Sato, Masahiro, Shindo, Takayuki
Format: Artigo
Sprache:Inglês
Veröffentlicht: BioMed Central 2014
Schlagworte:
Methodology Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4118159/
https://ncbi.nlm.nih.gov/pubmed/25042988
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-14-69
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