Direct Injection of CRISPR/Cas9-Related mRNA into Cytoplasm of Parthenogenetically Activated Porcine Oocytes Causes Frequent Mosaicism for Indel Mutations

Some reports demonstrated successful genome editing in pigs by one-step zygote microinjection of mRNA of CRISPR/Cas9-related components. Given the relatively long gestation periods and the high cost of housing, the establishment of a single blastocyst-based assay for rapid optimization of the above...

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Bibliographic Details
Published in:Int J Mol Sci
Main Authors: Sato, Masahiro, Koriyama, Miyu, Watanabe, Satoshi, Ohtsuka, Masato, Sakurai, Takayuki, Inada, Emi, Saitoh, Issei, Nakamura, Shingo, Miyoshi, Kazuchika
Format: Artigo
Language:Inglês
Published: MDPI 2015
Subjects:
Communication
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4581225/
https://ncbi.nlm.nih.gov/pubmed/26247938
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms160817838
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