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A non-inheritable maternal Cas9-based multiple-gene editing system in mice

The CRISPR/Cas9 system is capable of editing multiple genes through one-step zygote injection. The preexisting method is largely based on the co-injection of Cas9 DNA (or mRNA) and guide RNAs (gRNAs); however, it is unclear how many genes can be simultaneously edited by this method, and a reliable m...

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Bibliografiske detaljer
Udgivet i:Sci Rep
Main Authors: Sakurai, Takayuki, Kamiyoshi, Akiko, Kawate, Hisaka, Mori, Chie, Watanabe, Satoshi, Tanaka, Megumu, Uetake, Ryuichi, Sato, Masahiro, Shindo, Takayuki
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group 2016
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4730228/
https://ncbi.nlm.nih.gov/pubmed/26817415
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep20011
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