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Targeted Toxin-Based Selectable Drug-Free Enrichment of Mammalian Cells with High Transgene Expression
Almost all transfection protocols for mammalian cells use a drug resistance gene for the selection of transfected cells. However, it always requires the characterization of each isolated clone regarding transgene expression, which is time-consuming and labor-intensive. In the current study, we devel...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MDPI
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4009860/ https://ncbi.nlm.nih.gov/pubmed/24832665 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/biology2010341 |
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