Pooled shRNA Screen for Reactivation of MeCP2 on the Inactive X Chromosome

Forward genetic screens using reporter genes inserted into the heterochromatin have been extensively used to investigate mechanisms of epigenetic control in model organisms. Technologies including short hairpin RNAs (shRNAs) and clustered regularly interspaced short palindromic repeats (CRISPR) have...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Leko, Vid, Sripathy, Smitha, Adrianse, Robin L., Loe, Taylor, Park, Angela, Lao, Uyen, Foss, Eric J., Bartolomei, Marisa S., Bedalov, Antonio
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2018
Assuntos:
Genetics
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5931421/
https://ncbi.nlm.nih.gov/pubmed/29553562
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/56398
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