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Pooled shRNA Screen for Reactivation of MeCP2 on the Inactive X Chromosome

Forward genetic screens using reporter genes inserted into the heterochromatin have been extensively used to investigate mechanisms of epigenetic control in model organisms. Technologies including short hairpin RNAs (shRNAs) and clustered regularly interspaced short palindromic repeats (CRISPR) have...

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Dettagli Bibliografici
Pubblicato in:J Vis Exp
Autori principali: Leko, Vid, Sripathy, Smitha, Adrianse, Robin L., Loe, Taylor, Park, Angela, Lao, Uyen, Foss, Eric J., Bartolomei, Marisa S., Bedalov, Antonio
Natura: Artigo
Lingua:Inglês
Pubblicazione: MyJove Corporation 2018
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5931421/
https://ncbi.nlm.nih.gov/pubmed/29553562
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/56398
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