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Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases

It has been reported that mitochondria can contain up to 12 enzymatic sources of reactive oxygen species (ROS). A majority of these sites include flavin-dependent respiratory complexes and dehydrogenases that produce a mixture of superoxide (O(2)(●-)) and hydrogen peroxide (H(2)O(2)). Accurate quant...

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Dades bibliogràfiques
Publicat a:J Vis Exp
Autors principals: Mailloux, Ryan J., Young, Adrian, O'Brien, Marisa, Gill, Robert Morris
Format: Artigo
Idioma:Inglês
Publicat: MyJove Corporation 2018
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5931358/
https://ncbi.nlm.nih.gov/pubmed/29553554
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/56975
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