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Enhanced proofreading governs CRISPR-Cas9 targeting accuracy
The RNA-guided CRISPR-Cas9 nuclease from Streptococcus pyogenes (SpCas9) has been widely repurposed for genome editing(1–4). High-fidelity (SpCas9-HF1) and enhanced specificity (eSpCas9(1.1)) variants exhibit substantially reduced off-target cleavage in human cells, but the mechanism of target discr...
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| Veröffentlicht in: | Nature |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2017
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5918688/ https://ncbi.nlm.nih.gov/pubmed/28931002 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nature24268 |
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