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Using CRISPR/Cas9 Gene Editing to Investigate the Oncogenic Activity of Mutant Calreticulin in Cytokine Dependent Hematopoietic Cells

Clustered regularly interspaced short palindromic repeats (CRISPR) is an adaptive immunity system in prokaryotes that has been repurposed by scientists to generate RNA-guided nucleases, such as CRISPR-associated (Cas) 9 for site-specific eukaryotic genome editing. Genome engineering by Cas9 is used...

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Bibliografische gegevens
Gepubliceerd in:J Vis Exp
Hoofdauteurs: Abdelfattah, Nouran S., Mullally, Ann
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: MyJove Corporation 2018
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5908454/
https://ncbi.nlm.nih.gov/pubmed/29364275
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/56726
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