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Using CRISPR/Cas9 Gene Editing to Investigate the Oncogenic Activity of Mutant Calreticulin in Cytokine Dependent Hematopoietic Cells

Clustered regularly interspaced short palindromic repeats (CRISPR) is an adaptive immunity system in prokaryotes that has been repurposed by scientists to generate RNA-guided nucleases, such as CRISPR-associated (Cas) 9 for site-specific eukaryotic genome editing. Genome engineering by Cas9 is used...

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Vydáno v:J Vis Exp
Hlavní autoři: Abdelfattah, Nouran S., Mullally, Ann
Médium: Artigo
Jazyk:Inglês
Vydáno: MyJove Corporation 2018
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5908454/
https://ncbi.nlm.nih.gov/pubmed/29364275
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/56726
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