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Using CRISPR/Cas9 Gene Editing to Investigate the Oncogenic Activity of Mutant Calreticulin in Cytokine Dependent Hematopoietic Cells

Clustered regularly interspaced short palindromic repeats (CRISPR) is an adaptive immunity system in prokaryotes that has been repurposed by scientists to generate RNA-guided nucleases, such as CRISPR-associated (Cas) 9 for site-specific eukaryotic genome editing. Genome engineering by Cas9 is used...

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Bibliografske podrobnosti
izdano v:J Vis Exp
Main Authors: Abdelfattah, Nouran S., Mullally, Ann
Format: Artigo
Jezik:Inglês
Izdano: MyJove Corporation 2018
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC5908454/
https://ncbi.nlm.nih.gov/pubmed/29364275
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/56726
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