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CRISPR/Cas9 genome editing in human hematopoietic stem cells

Genome editing via homologous recombination (HR) (gene targeting) in human hematopoietic stem cells (HSCs) has the power to reveal gene–function relationships and potentially transform curative hematological gene and cell therapies. However, there are no comprehensive and reproducible protocols for...

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Detalles Bibliográficos
Publicado en:Nat Protoc
Main Authors: Bak, Rasmus O, Dever, Daniel P, Porteus, Matthew H
Formato: Artigo
Idioma:Inglês
Publicado: 2018
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5826598/
https://ncbi.nlm.nih.gov/pubmed/29370156
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2017.143
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