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Incorporation of Phosphorylated Tyrosine into Proteins: In Vitro Translation and Study of Phosphorylated IκB-α and Its Interaction with NF-κB

Phosphorylated proteins play important roles in the regulation of many different cell networks. However, unlike the preparation of proteins containing unmodiffed proteinogenic amino acids, which can be altered readily by site-directed mutagenesis and expressed in vitro and in vivo, the preparation o...

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Détails bibliographiques
Publié dans:J Am Chem Soc
Auteurs principaux: Chen, Shengxi, Maini, Rumit, Bai, Xiaoguang, Nangreave, Ryan C., Dedkova, Larisa M., Hecht, Sidney M.
Format: Artigo
Langue:Inglês
Publié: 2017
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5901656/
https://ncbi.nlm.nih.gov/pubmed/28898075
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jacs.7b05168
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