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Incorporation of Phosphorylated Tyrosine into Proteins: In Vitro Translation and Study of Phosphorylated IκB-α and Its Interaction with NF-κB

Phosphorylated proteins play important roles in the regulation of many different cell networks. However, unlike the preparation of proteins containing unmodiffed proteinogenic amino acids, which can be altered readily by site-directed mutagenesis and expressed in vitro and in vivo, the preparation o...

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Detalles Bibliográficos
Publicado en:J Am Chem Soc
Main Authors: Chen, Shengxi, Maini, Rumit, Bai, Xiaoguang, Nangreave, Ryan C., Dedkova, Larisa M., Hecht, Sidney M.
Formato: Artigo
Idioma:Inglês
Publicado: 2017
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5901656/
https://ncbi.nlm.nih.gov/pubmed/28898075
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jacs.7b05168
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