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Comparison and optimization of CRISPR/dCas9/gRNA genome-labeling systems for live cell imaging
CRISPR/dCas9 binds precisely to defined genomic sequences through targeting of guide RNA (gRNA) sequences. In vivo imaging of genomic loci can be achieved by recruiting fluorescent proteins using either dCas9 or gRNA. We thoroughly validate and compare the effectiveness and specificity of several dC...
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| Publicado no: | Genome Biol |
|---|---|
| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5863892/ https://ncbi.nlm.nih.gov/pubmed/29566733 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-018-1413-5 |
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