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Sickle Cell Imaging Flow Cytometry Assay (SIFCA)

Hemoglobin S polymerization under hypoxic conditions in sickle cell disorders causes characteristic shape changes to human red blood cells. Previous sickling assays used to investigate the efficacy of novel agents to treat these disorders are laborious and observer dependent. Here, we describe a par...

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Detalles Bibliográficos
Publicado en:Methods Mol Biol
Main Authors: Fertrin, Kleber Y., Samsel, Leigh, van Beers, Eduard J., Mendelsohn, Laurel, Kato, Gregory J., McCoy, J. Philip
Formato: Artigo
Idioma:Inglês
Publicado: 2016
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5826554/
https://ncbi.nlm.nih.gov/pubmed/27460253
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-3302-0_20
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