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Profiling the transcriptome by RNA SPOTs

Single molecule FISH (smFISH) has been the gold standard in quantifying individual transcripts abundances. Here, we demonstrate the scaling up of smFISH to the transcriptome level by profiling of 10,212 different mRNAs from mouse fibroblast and embryonic stem cells. This methods, called RNA SPOTs (S...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Nat Methods
Prif Awduron: Eng, Chee-Huat Linus, Shah, Sheel, Thomassie, Julian, Cai, Long
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: 2017
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC5819366/
https://ncbi.nlm.nih.gov/pubmed/29131163
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.4500
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