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Profiling the transcriptome by RNA SPOTs
Single molecule FISH (smFISH) has been the gold standard in quantifying individual transcripts abundances. Here, we demonstrate the scaling up of smFISH to the transcriptome level by profiling of 10,212 different mRNAs from mouse fibroblast and embryonic stem cells. This methods, called RNA SPOTs (S...
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| Veröffentlicht in: | Nat Methods |
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| Hauptverfasser: | , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2017
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5819366/ https://ncbi.nlm.nih.gov/pubmed/29131163 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.4500 |
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