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Profiling the transcriptome by RNA SPOTs

Single molecule FISH (smFISH) has been the gold standard in quantifying individual transcripts abundances. Here, we demonstrate the scaling up of smFISH to the transcriptome level by profiling of 10,212 different mRNAs from mouse fibroblast and embryonic stem cells. This methods, called RNA SPOTs (S...

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Detalhes bibliográficos
Publicado no:Nat Methods
Main Authors: Eng, Chee-Huat Linus, Shah, Sheel, Thomassie, Julian, Cai, Long
Formato: Artigo
Idioma:Inglês
Publicado em: 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5819366/
https://ncbi.nlm.nih.gov/pubmed/29131163
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.4500
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