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Profiling the transcriptome by RNA SPOTs

Single molecule FISH (smFISH) has been the gold standard in quantifying individual transcripts abundances. Here, we demonstrate the scaling up of smFISH to the transcriptome level by profiling of 10,212 different mRNAs from mouse fibroblast and embryonic stem cells. This methods, called RNA SPOTs (S...

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書目詳細資料
發表在:Nat Methods
Main Authors: Eng, Chee-Huat Linus, Shah, Sheel, Thomassie, Julian, Cai, Long
格式: Artigo
語言:Inglês
出版: 2017
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC5819366/
https://ncbi.nlm.nih.gov/pubmed/29131163
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.4500
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