Dynamics and spatial genomics of the nascent transcriptome by intron seqFISH

Visualization of the transcriptome and the nuclear organization in situ has been challenging for single cell analysis. Here, we demonstrate a multiplexed single molecule in situ method, intron seqFISH, that allows imaging of 10,421 genes at their nascent transcription active sites in single cells, f...

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發表在:Cell
Main Authors: Shah, Sheel, Takei, Yodai, Zhou, Wen, Lubeck, Eric, Yun, Jina, Eng, Chee-Huat Linus, Koulena, Noushin, Cronin, Christopher, Karp, Christoph, Liaw, Eric J., Amin, Mina, Cai, Long
格式: Artigo
語言:Inglês
出版: 2018
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Article
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC6046268/
https://ncbi.nlm.nih.gov/pubmed/29887381
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.cell.2018.05.035
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