Refined sgRNA efficacy prediction improves large- and small-scale CRISPR–Cas9 applications

Genome editing with the CRISPR–Cas9 system has enabled unprecedented efficacy for reverse genetics and gene correction approaches. While off-target effects have been successfully tackled, the effort to eliminate variability in sgRNA efficacies—which affect experimental sensitivity—is in its infancy....

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Salvato in:
Dettagli Bibliografici
Pubblicato in:Nucleic Acids Res
Autori principali: Labuhn, Maurice, Adams, Felix F, Ng, Michelle, Knoess, Sabine, Schambach, Axel, Charpentier, Emmanuelle M, Schwarzer, Adrian, Mateo, Juan L, Klusmann, Jan-Henning, Heckl, Dirk
Natura: Artigo
Lingua:Inglês
Pubblicazione: Oxford University Press 2018
Soggetti:
Methods Online
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5814880/
https://ncbi.nlm.nih.gov/pubmed/29267886
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkx1268
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