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A new strategy of using O(18)-labeled iodoacetic acid for mass spectrometry-based protein quantitation
A new O(18) labeling protocol is designed to assist quantitation of cysteine-containing proteins using LC/MS. Unlike other O(18) labeling strategies, the labeling is carried out at the intact protein level (prior to its digestion) during reduction/alkylation of cysteine side chains using O(18)-label...
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| Udgivet i: | J Am Soc Mass Spectrom |
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| Main Authors: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2012
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5809132/ https://ncbi.nlm.nih.gov/pubmed/22562395 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s13361-012-0396-9 |
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