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A new strategy of using O(18)-labeled iodoacetic acid for mass spectrometry-based protein quantitation

A new O(18) labeling protocol is designed to assist quantitation of cysteine-containing proteins using LC/MS. Unlike other O(18) labeling strategies, the labeling is carried out at the intact protein level (prior to its digestion) during reduction/alkylation of cysteine side chains using O(18)-label...

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Detalhes bibliográficos
Publicado no:J Am Soc Mass Spectrom
Main Authors: Wang, Shunhai, Kaltashov, Igor A.
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5809132/
https://ncbi.nlm.nih.gov/pubmed/22562395
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s13361-012-0396-9
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