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RNA-dependent RNA targeting by CRISPR-Cas9

Double-stranded DNA (dsDNA) binding and cleavage by Cas9 is a hallmark of type II CRISPR-Cas bacterial adaptive immunity. All known Cas9 enzymes are thought to recognize DNA exclusively as a natural substrate, providing protection against DNA phage and plasmids. Here, we show that Cas9 enzymes from...

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Bibliographische Detailangaben
Veröffentlicht in:eLife
Hauptverfasser: Strutt, Steven C, Torrez, Rachel M, Kaya, Emine, Negrete, Oscar A, Doudna, Jennifer A
Format: Artigo
Sprache:Inglês
Veröffentlicht: eLife Sciences Publications, Ltd 2018
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5796797/
https://ncbi.nlm.nih.gov/pubmed/29303478
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.32724
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