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RNA-dependent RNA targeting by CRISPR-Cas9

Double-stranded DNA (dsDNA) binding and cleavage by Cas9 is a hallmark of type II CRISPR-Cas bacterial adaptive immunity. All known Cas9 enzymes are thought to recognize DNA exclusively as a natural substrate, providing protection against DNA phage and plasmids. Here, we show that Cas9 enzymes from...

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Detalhes bibliográficos
Publicado no:eLife
Main Authors: Strutt, Steven C, Torrez, Rachel M, Kaya, Emine, Negrete, Oscar A, Doudna, Jennifer A
Formato: Artigo
Idioma:Inglês
Publicado em: eLife Sciences Publications, Ltd 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5796797/
https://ncbi.nlm.nih.gov/pubmed/29303478
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.32724
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