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Nucleosome breathing and remodeling constrain CRISPR-Cas9 function

The CRISPR-Cas9 bacterial surveillance system has become a versatile tool for genome editing and gene regulation in eukaryotic cells, yet how CRISPR-Cas9 contends with the barriers presented by eukaryotic chromatin is poorly understood. Here we investigate how the smallest unit of chromatin, a nucle...

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Detalhes bibliográficos
Publicado no:eLife
Main Authors: Isaac, R Stefan, Jiang, Fuguo, Doudna, Jennifer A, Lim, Wendell A, Narlikar, Geeta J, Almeida, Ricardo
Formato: Artigo
Idioma:Inglês
Publicado em: eLife Sciences Publications, Ltd 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4880442/
https://ncbi.nlm.nih.gov/pubmed/27130520
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.13450
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