Improving CRISPR–Cas specificity with chemical modifications in single-guide RNAs

CRISPR systems have emerged as transformative tools for altering genomes in living cells with unprecedented ease, inspiring keen interest in increasing their specificity for perfectly matched targets. We have developed a novel approach for improving specificity by incorporating chemical modification...

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Bibliografische gegevens
Gepubliceerd in:Nucleic Acids Res
Hoofdauteurs: Ryan, Daniel E, Taussig, David, Steinfeld, Israel, Phadnis, Smruti M, Lunstad, Benjamin D, Singh, Madhurima, Vuong, Xuan, Okochi, Kenji D, McCaffrey, Ryan, Olesiak, Magdalena, Roy, Subhadeep, Yung, Chong Wing, Curry, Bo, Sampson, Jeffrey R, Bruhn, Laurakay, Dellinger, Douglas J
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Oxford University Press 2018
Onderwerpen:
Molecular Biology
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5778453/
https://ncbi.nlm.nih.gov/pubmed/29216382
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkx1199
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