Improving CRISPR–Cas specificity with chemical modifications in single-guide RNAs

CRISPR systems have emerged as transformative tools for altering genomes in living cells with unprecedented ease, inspiring keen interest in increasing their specificity for perfectly matched targets. We have developed a novel approach for improving specificity by incorporating chemical modification...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Ryan, Daniel E, Taussig, David, Steinfeld, Israel, Phadnis, Smruti M, Lunstad, Benjamin D, Singh, Madhurima, Vuong, Xuan, Okochi, Kenji D, McCaffrey, Ryan, Olesiak, Magdalena, Roy, Subhadeep, Yung, Chong Wing, Curry, Bo, Sampson, Jeffrey R, Bruhn, Laurakay, Dellinger, Douglas J
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2018
Assuntos:
Molecular Biology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5778453/
https://ncbi.nlm.nih.gov/pubmed/29216382
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkx1199
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