Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells

CRISPR-Cas-mediated genome editing relies on guide RNAs that direct site-specific DNA cleavage facilitated by the Cas endonuclease. Here we report that chemical alterations to synthesized single guide RNAs (sgRNAs) enhance genome editing efficiency in human primary T cells and CD34(+) hematopoietic...

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Publicat a:Nat Biotechnol
Autors principals: Hendel, Ayal, Bak, Rasmus O, Clark, Joseph T, Kennedy, Andrew B, Ryan, Daniel E, Roy, Subhadeep, Steinfeld, Israel, Lunstad, Benjamin D, Kaiser, Robert J, Wilkens, Alec B, Bacchetta, Rosa, Tsalenko, Anya, Dellinger, Douglas, Bruhn, Laurakay, Porteus, Matthew H
Format: Artigo
Idioma:Inglês
Publicat: 2015
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Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4729442/
https://ncbi.nlm.nih.gov/pubmed/26121415
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.3290
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