Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells

CRISPR-Cas-mediated genome editing relies on guide RNAs that direct site-specific DNA cleavage facilitated by the Cas endonuclease. Here we report that chemical alterations to synthesized single guide RNAs (sgRNAs) enhance genome editing efficiency in human primary T cells and CD34(+) hematopoietic...

Täydet tiedot

Tallennettuna:
Bibliografiset tiedot
Julkaisussa:Nat Biotechnol
Päätekijät: Hendel, Ayal, Bak, Rasmus O, Clark, Joseph T, Kennedy, Andrew B, Ryan, Daniel E, Roy, Subhadeep, Steinfeld, Israel, Lunstad, Benjamin D, Kaiser, Robert J, Wilkens, Alec B, Bacchetta, Rosa, Tsalenko, Anya, Dellinger, Douglas, Bruhn, Laurakay, Porteus, Matthew H
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: 2015
Aiheet:
Article
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC4729442/
https://ncbi.nlm.nih.gov/pubmed/26121415
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.3290
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