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Method to Assemble Genomic DNA Fragments or Genes on Human Artificial Chromosome with Regulated Kinetochore Using a Multi-Integrase System

[Image: see text] The production of cells capable of carrying multiple transgenes to Mb-size genomic loci has multiple applications in biomedicine and biotechnology. In order to achieve this goal, three key steps are required: (i) cloning of large genomic segments; (ii) insertion of multiple DNA blo...

Πλήρης περιγραφή

Αποθηκεύτηκε σε:
Λεπτομέρειες βιβλιογραφικής εγγραφής
Τόπος έκδοσης:ACS Synth Biol
Κύριοι συγγραφείς: Lee, Nicholas C. O., Kim, Jung-Hyun, Petrov, Nikolai S., Lee, Hee-Sheung, Masumoto, Hiroshi, Earnshaw, William C., Larionov, Vladimir, Kouprina, Natalay
Μορφή: Artigo
Γλώσσα:Inglês
Έκδοση: American Chemical Society 2017
Διαθέσιμο Online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5778389/
https://ncbi.nlm.nih.gov/pubmed/28799737
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acssynbio.7b00209
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