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Single mRNA molecule detection in Drosophila
Single molecule fluorescent in situ hybridization (smFISH) enables quantitative measurements of gene expression and mRNA localization. The technique is increasingly popular for analysis of cultured cells but is not widely applied to intact organisms. Here we describe a method for labeling and detect...
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| Publicat a: | Methods Mol Biol |
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| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2018
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5772605/ https://ncbi.nlm.nih.gov/pubmed/29130194 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-7213-5_8 |
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