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mRNA quantification using single-molecule FISH in Drosophila embryos
Spatial information is crucial to interrogate developmental and tissue-level regulation of gene expression; this information is usually lost when mRNA levels from the entire tissue are measured using reverse transcriptase PCR, microarray or high-throughput sequencing. In contrast, single-molecule fl...
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| Veröffentlicht in: | Nat Protoc |
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| Hauptverfasser: | , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2016
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6668020/ https://ncbi.nlm.nih.gov/pubmed/28594816 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2017.030 |
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