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Single mRNA molecule detection in Drosophila

Single molecule fluorescent in situ hybridization (smFISH) enables quantitative measurements of gene expression and mRNA localization. The technique is increasingly popular for analysis of cultured cells but is not widely applied to intact organisms. Here we describe a method for labeling and detect...

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Bibliografische gegevens
Gepubliceerd in:Methods Mol Biol
Hoofdauteurs: Little, Shawn C., Gregor, Thomas
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 2018
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5772605/
https://ncbi.nlm.nih.gov/pubmed/29130194
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-7213-5_8
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