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Allele-specific detection of single mRNA molecules in situ

We describe a method for fluorescent in situ identification of individual mRNA molecules, allowing quantitative and accurate measurements of allele-specific transcripts that differ by only a few nucleotides, in single cells. By using a combination of allele-specific and non-allele-specific probe lib...

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Detalhes bibliográficos
Main Authors: Hansen, Clinton H., van Oudenaarden, Alexander
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3789122/
https://ncbi.nlm.nih.gov/pubmed/23934076
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.2601
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