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Imaging individual mRNA molecules using multiple singly labeled probes
We describe a method for imaging individual mRNA molecules in fixed cells by probing each mRNA species with 48 or more short, singly labeled oligonucleotide probes. This makes each mRNA molecule visible as a computationally identifiable fluorescent spot via fluorescence microscopy. We demonstrate si...
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| Autores principales: | , , , , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
2008
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3126653/ https://ncbi.nlm.nih.gov/pubmed/18806792 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1253 |
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