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Allele-specific detection of single mRNA molecules in situ
We describe a method for fluorescent in situ identification of individual mRNA molecules, allowing quantitative and accurate measurements of allele-specific transcripts that differ by only a few nucleotides, in single cells. By using a combination of allele-specific and non-allele-specific probe lib...
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| Main Authors: | , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2013
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3789122/ https://ncbi.nlm.nih.gov/pubmed/23934076 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.2601 |
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