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mRNA quantification using single-molecule FISH in Drosophila embryos

Spatial information is crucial to interrogate developmental and tissue-level regulation of gene expression; this information is usually lost when mRNA levels from the entire tissue are measured using reverse transcriptase PCR, microarray or high-throughput sequencing. In contrast, single-molecule fl...

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Bibliografiska uppgifter
I publikationen:Nat Protoc
Huvudupphovsmän: Trcek, Tatjana, Lionnet, Timothée, Shroff, Hari, Lehmann, Ruth
Materialtyp: Artigo
Språk:Inglês
Publicerad: 2016
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC6668020/
https://ncbi.nlm.nih.gov/pubmed/28594816
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2017.030
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