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Generation of Recombinant Vaccinia Viruses

This unit describes how to infect cells with vaccinia virus and then transfect them with a plasmid-transfer vector or PCR fragment to generate a recombinant virus. Selection and screening methods used to isolate recombinant viruses and a method for the amplification of recombinant viruses are descri...

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Bibliografiske detaljer
Udgivet i:Curr Protoc Protein Sci
Main Authors: Earl, Patricia L., Moss, Bernard, Wyatt, Linda S.
Format: Artigo
Sprog:Inglês
Udgivet: 2017
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5765993/
https://ncbi.nlm.nih.gov/pubmed/28762491
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpps.33
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