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UNIT 14A.4 Generation of Recombinant Vaccinia Viruses
This unit describes how to infect cells with vaccinia virus and then transfect them with a plasmid-transfer vector or PCR fragment to generate a recombinant virus. Selection and screening methods used to isolate recombinant viruses and a method for the amplification of recombinant viruses are descri...
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| 發表在: | Curr Protoc Microbiol |
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| Main Authors: | , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
2015
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5123791/ https://ncbi.nlm.nih.gov/pubmed/26528782 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/9780471729259.mc14a04s39 |
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