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Preparation of Cell Cultures and Vaccinia Virus Stocks

The culturing of cell lines used with vaccinia virus, both as monolayer and in suspension, is described. The preparation of chick embryo fibroblasts (CEF) is presented for use in the production of the highly attenuated and host range-restricted modified vaccinia virus Ankara (MVA) strain of vaccinia...

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Detalhes bibliográficos
Publicado no:Curr Protoc Protein Sci
Main Authors: Cotter, Catherine A, Earl, Patricia L, Wyatt, Linda S, Moss, Bernard
Formato: Artigo
Idioma:Inglês
Publicado em: 2001
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5575744/
https://ncbi.nlm.nih.gov/pubmed/18429178
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/0471140864.ps0512s13
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