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Preparation of Cell Cultures and Vaccinia Virus Stocks
The culturing of cell lines used with vaccinia virus, both as monolayer and in suspension, is described. The preparation of chick embryo fibroblasts (CEF) is presented for use in the production of the highly attenuated and host range-restricted modified vaccinia virus Ankara (MVA) strain of vaccinia...
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| Publicado en: | Curr Protoc Protein Sci |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2001
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5575744/ https://ncbi.nlm.nih.gov/pubmed/18429178 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/0471140864.ps0512s13 |
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