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Preparation of Cell Cultures and Vaccinia Virus Stocks

The culturing of cell lines used with vaccinia virus, both as monolayer and in suspension, is described. The preparation of chick embryo fibroblasts (CEF) is presented for use in the production of the highly attenuated and host range-restricted modified vaccinia virus Ankara (MVA) strain of vaccinia...

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Détails bibliographiques
Publié dans:Curr Protoc Protein Sci
Auteurs principaux: Cotter, Catherine A, Earl, Patricia L, Wyatt, Linda S, Moss, Bernard
Format: Artigo
Langue:Inglês
Publié: 2001
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5575744/
https://ncbi.nlm.nih.gov/pubmed/18429178
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/0471140864.ps0512s13
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