Stimulation of reverse transcriptase generated cDNAs with specific indels by template RNA structure: retrotransposon, dNTP balance, RT-reagent usage

RNA dependent DNA-polymerases, reverse transcriptases, are key enzymes for retroviruses and retroelements. Their fidelity, including indel generation, is significant for their use as reagents including for deep sequencing. Here, we report that certain RNA template structures and G-rich sequences, ah...

Descrizione completa

Salvato in:
Dettagli Bibliografici
Pubblicato in:Nucleic Acids Res
Autori principali: Penno, Christophe, Kumari, Romika, Baranov, Pavel V., van Sinderen, Douwe, Atkins, John F.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Oxford University Press 2017
Soggetti:
Molecular Biology
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5737552/
https://ncbi.nlm.nih.gov/pubmed/28973469
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkx689
Tags: Aggiungi Tag
Nessun Tag, puoi essere il primo ad aggiungerne! !

Documenti analoghi