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HIV-1 Reverse Transcriptase based assay to determine cellular dNTP concentrations

Deoxynucleoside triphosphates (dNTPs) are the building blocks of DNA and their biosynthesis are tightly regulated in the cell. HPLC-MS and enzyme-based methods are currently employed to determine dNTP concentrations from cellular extracts. Here, we describe a highly efficient, HIV-1 reverse transcri...

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Publicat a:Methods Mol Biol
Autors principals: Hollenbaugh, Joseph A., Kim, Baek
Format: Artigo
Idioma:Inglês
Publicat: 2016
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4839283/
https://ncbi.nlm.nih.gov/pubmed/26714705
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-3046-3_5
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