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HIV-1 Reverse Transcriptase based assay to determine cellular dNTP concentrations
Deoxynucleoside triphosphates (dNTPs) are the building blocks of DNA and their biosynthesis are tightly regulated in the cell. HPLC-MS and enzyme-based methods are currently employed to determine dNTP concentrations from cellular extracts. Here, we describe a highly efficient, HIV-1 reverse transcri...
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| Published in: | Methods Mol Biol |
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| Main Authors: | , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
2016
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4839283/ https://ncbi.nlm.nih.gov/pubmed/26714705 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-3046-3_5 |
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