Stimulation of reverse transcriptase generated cDNAs with specific indels by template RNA structure: retrotransposon, dNTP balance, RT-reagent usage

RNA dependent DNA-polymerases, reverse transcriptases, are key enzymes for retroviruses and retroelements. Their fidelity, including indel generation, is significant for their use as reagents including for deep sequencing. Here, we report that certain RNA template structures and G-rich sequences, ah...

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Detaylı Bibliyografya
Yayımlandı:Nucleic Acids Res
Asıl Yazarlar: Penno, Christophe, Kumari, Romika, Baranov, Pavel V., van Sinderen, Douwe, Atkins, John F.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Oxford University Press 2017
Konular:
Molecular Biology
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC5737552/
https://ncbi.nlm.nih.gov/pubmed/28973469
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkx689
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