Red-emitting chimeric firefly luciferase for in vivo imaging in low ATP cellular environments

Beetle luciferases have been adapted for live cell imaging where bioluminescence is dependent on the cellular availability of ATP, O(2), and added luciferin. Previous Photinus pyralis red-emitting variants with high K(m) values for ATP have performed disappointingly in live cells despite having much...

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Vydáno v:Anal Biochem
Hlavní autoři: Branchini, Bruce R., Southworth, Tara L., Fontaine, Danielle M., Kohrt, Dawn, Welcome, Franceine S., Florentine, Catherine M., Henricks, Emma R., DeBartolo, Demetria B., Michelini, Elisa, Cevenini, Luca, Roda, Aldo, Grossel, Martha J.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2017
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5730985/
https://ncbi.nlm.nih.gov/pubmed/28687486
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2017.07.001
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