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Red-emitting chimeric firefly luciferase for in vivo imaging in low ATP cellular environments

Beetle luciferases have been adapted for live cell imaging where bioluminescence is dependent on the cellular availability of ATP, O(2), and added luciferin. Previous Photinus pyralis red-emitting variants with high K(m) values for ATP have performed disappointingly in live cells despite having much...

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Detalhes bibliográficos
Publicado no:Anal Biochem
Main Authors: Branchini, Bruce R., Southworth, Tara L., Fontaine, Danielle M., Kohrt, Dawn, Welcome, Franceine S., Florentine, Catherine M., Henricks, Emma R., DeBartolo, Demetria B., Michelini, Elisa, Cevenini, Luca, Roda, Aldo, Grossel, Martha J.
Formato: Artigo
Idioma:Inglês
Publicado em: 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5730985/
https://ncbi.nlm.nih.gov/pubmed/28687486
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2017.07.001
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